CENPE Antibody (PSH03-54)

Western Blot: CENPE Antibody (PSH03-54) [NBP3-32180]

Western Blot: CENPE Antibody (PSH03-54) [NBP3-32180] - Western blot analysis of CENPE on different lysates with Rabbit anti-CENPE antibody (NBP3-32180) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: HEK-293 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 316 kDa
Observed band size: 316 kDa

Exposure time: 43 seconds;

3-8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32180) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.