CENPF Antibody (7F11.2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13730

Novus Biologicals, part of Bio-Techne
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NBP3-13730
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 7F11.2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CENPF Antibodies »

Product Summary for CENPF Antibody (7F11.2)

Immunogen

Protein fragment expressed in E. coli corresponding to amino acids 1759-2093.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

358 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for CENPF Antibody (7F11.2)

Immunocytochemistry/ Immunofluorescence: CENPF Antibody (7F11.2) [NBP3-13730]

Immunocytochemistry/ Immunofluorescence: CENPF Antibody (7F11.2) [NBP3-13730]

Immunocytochemistry/Immunofluorescence: CENPF Antibody (7F11.2) [NBP3-13730] - Immunofluorescent staining of mitotic U2OS cells with mitosin/CENP-F antibody NBP3-13730 (green) at 1:500 dilution. Cells were pre-extracted with ice-cold CSK buffer (0.5% Triton X-100, 300 mM Sucrose, 10 mM PIPES, pH8.0) for 4 min, followed by fixation with 4% PFA and regular IFA procedure. Blue channel (DAPI staining) shows chromosomes.
Immunohistochemistry: CENPF Antibody (7F11.2) [NBP3-13730]

Immunohistochemistry: CENPF Antibody (7F11.2) [NBP3-13730]

Immunohistochemistry: CENPF Antibody (7F11.2) [NBP3-13730] - High magnification photomicrograph of an NG3 tumor with a high percentage of mitosin-positive nuclei, indicative of high proliferative activity.

Applications for CENPF Antibody (7F11.2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

10 g/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CENPF

CENPF (Centromere protein F) is required for proper chromosome segregation in mitosis, and has a cell-cycle distribution that is both temporally regulated and diverse in terms of the structural component localization. Specifically, CENPF is a component of the nuclear matrix during the G2 phase of interphase. In late G2, it associates with the kinetochore and maintains this association through early anaphase. Here, CENPF is required for kinetochore function and appears to be the earliest member to interact with the centromere-kinetochore complex. It then localizes to the spindle midzone in late anaphase and to the intracellular bridge in telophase, where the protein is subsequently degraded. Given this diversity of function, CENPF is expected to play an important role in several mitotic events.

Alternate Names

AH antigen, cell-cycle-dependent 350K nuclear protein, CENF, CENP-F, CENP-F kinetochore protein, centromere protein F, centromere protein F (350/400kD, mitosin), centromere protein F, 350/400ka (mitosin), centromere protein F, 350/400kDa (mitosin), hcp-1, Kinetochore protein CENPF, Mitosin, PRO1779

Gene Symbol

CENPF

Additional CENPF Products

Product Documents for CENPF Antibody (7F11.2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CENPF Antibody (7F11.2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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