CENPF Antibody (PSH0-79)

Western Blot: CENPF Antibody (PSH0-79) [NBP3-32181]

Western Blot: CENPF Antibody (PSH0-79) [NBP3-32181] - Western blot analysis of CENPF on different lysates with Rabbit anti-CENPF antibody (NBP3-32181) at 1/1,000 dilution.

Lane 1: SK-Br-3 cell lysate
Lane 2: Jurkat cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 358 kDa
Observed band size: 358 kDa

Exposure time: 3 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32181) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: CENPF Antibody (PSH0-79) [NBP3-32181]

Immunohistochemistry: CENPF Antibody (PSH0-79) [NBP3-32181] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CENPF antibody (NBP3-32181) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32181) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.