Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Bovine, Equine

Cited:

Human, Mouse, Bovine, Equine

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all CENPF Antibodies »

Product Specifications

Immunogen

A bacterial fusion protein from the C-terminus of human CENPF. [UniProt# P49454]

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CENPF Antibody - BSA Free

Knockdown Validated: CENPF Antibody - BSA Free [NB500-101]

Knockdown Validated: CENPF Antibody - BSA Free [NB500-101]

Knockdown Validated: CENPF Antibody - BSA Free [NB500-101] - Kinetochore localization of RZZ and MAD1 are independent of CENP-E and CENP-F. CENP-F depletion does not interfere with the recruitment of Zwilch. The graphs show mean intensity of one (B, C, and E), two (D and F), or three (A, G, and H) experiments; the error bars indicate S.E., and the mean values for nondepleted cells are set to 1. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29748388/) licensed under a CC-BY license.
Western Blot: CENPF AntibodyBSA Free [NB500-101]

Western Blot: CENPF AntibodyBSA Free [NB500-101]

Western Blot: CENPF Antibody [NB500-101] - 25ug of asynchronous and mitotically blocked HeLa lysates were loaded in each respective lane.
Immunocytochemistry/ Immunofluorescence: CENPF Antibody - BSA Free [NB500-101]

Immunocytochemistry/ Immunofluorescence: CENPF Antibody - BSA Free [NB500-101]

Immunocytochemistry/Immunofluorescence: CENPF Antibody [NB500-101] - Distribution of CENP-F (red), largely associated with the mitotic spindle and a clearly distinguishable frac.
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Applications for CENPF Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:200-1:1000immunoprecipitation 1:50

Immunohistochemistry Whole-Mount

reported in scientific literature (PMID 24876181)

Immunohistochemistry-Frozen

reported in scientific literature (PMID 26195156)

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 20398247)

Immunoprecipitation

1:50

Western Blot

1:1500-1:2500
Application Notes
In Western Blot, a band can be seen at ~330 kDa. In ICC/IF, nuclear staining was observed in HeLa cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NB500-101 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CENPF

CENPF interacts with the centromere-kinetochore complex. CENP-F also shows a cell-cycle distribution that is both temporally regulated and diverse in terms of the structural components it localizes to and therefore may play a role in several mitotic events.

Alternate Names

AH antigen, cell-cycle-dependent 350K nuclear protein, CENF, CENP-F, CENP-F kinetochore protein, centromere protein F, centromere protein F (350/400kD, mitosin), centromere protein F, 350/400ka (mitosin), centromere protein F, 350/400kDa (mitosin), hcp-1, Kinetochore protein CENPF, Mitosin, PRO1779

Gene Symbol

CENPF

Additional CENPF Products

Product Documents for CENPF Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CENPF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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