CENPH Antibody (1F7)

Novus Biologicals, part of Bio-Techne | Catalog # H00064946-M01

Novus Biologicals, part of Bio-Techne
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H00064946-M01
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CENPH Antibodies »

Product Specifications

Immunogen

CENPH (AAH12024, 1 a.a. ~ 247 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEEQPQMQDADEPADSGGEGRAGGPPQVAGAQAACSEDRMTLLLRLRAQTKQQLLEYKSMVDASEEKTPEQIMQEKQIEAKIEDLENEIEEVKVAFEIKKLALDRMRLSTALKKNLEKISRQSSVLMDNMKHLLELNKLIMKSQQESWDLEEKLLDIRKKRLQLKQASESKLLEIQTEKNKQKIDLDSMENSERIKIIRQNLQMEIKITTVIQHVFQNLILGSKVNWAEDPALKEIVLQLEKNVDMM

Specificity

CENPH - centromere protein H (1F7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CENPH Antibody (1F7)

Western Blot: CENPH Antibody (1F7) [H00064946-M01]

Western Blot: CENPH Antibody (1F7) [H00064946-M01]

Western Blot: CENPH Antibody (1F7) [H00064946-M01] - Analysis of CENPH expression in transfected 293T cell line by CENPH monoclonal antibody (M01), clone 1F7.Lane 1: CENPH transfected lysate(28.5 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: CENPH Antibody (1F7) [H00064946-M01] - Detection limit for recombinant GST tagged CENPH is 0.03 ng/ml as a capture antibody.

Applications for CENPH Antibody (1F7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CENPH

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq]

Alternate Names

CENP-H, centromere protein H, ICEN35, Interphase centromere complex protein 35, kinetochore protein CENP-H, NNF1, PMF1

Entrez Gene IDs

64946 (Human)

Gene Symbol

CENPH

UniProt

AAH12024 (Human)

Additional CENPH Products

Product Documents for CENPH Antibody (1F7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CENPH Antibody (1F7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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