Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CENPT Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 520 and 561 of human centromere protein T using the numbering given in entry NP_079358.3 (GeneID 80152).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CENPT Antibody

Immunoprecipitation: CENPT Antibody [NBP1-28698]

Immunoprecipitation: CENPT Antibody [NBP1-28698]

Immunoprecipitation: CENPT Antibody [NBP1-28698] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CENP-T antibody NBP1-28698 used for IP at 10 ug/mg lysate. CENP-T was also immunoprecipitated by rabbit anti-CENP-T antibody NBP1-28744, which recognizes an upstream epitope. For blotting immunoprecipitated CENP-T, NBP1-28744 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for CENPT Antibody

Application
Recommended Usage

Immunoprecipitation

10 ug/mg lysate
Application Notes
It is not recommended for western analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CENPT

Centromere protein T (CENP-T) is a component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENP-T is one of the basic inner kinetochore proteins with most further proteins binding downstream, suggesting a fundamental role of CENP-T in kinetochore function.

Alternate Names

C16orf56, CENP-Tchromosome 16 open reading frame 56, centromere protein T, FLJ13111, FLJ43376, ICEN22, Interphase centromere complex protein 22

Entrez Gene IDs

80152 (Human)

Gene Symbol

CENPT

UniProt

NP_079358.3 (Human)

Additional CENPT Products

Product Documents for CENPT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CENPT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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