Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CENPT Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 480 and 520 of human centromere protein T using the numbering given in entry NP_079358.3 (GeneID 80152).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CENPT Antibody

Western Blot: CENPT Antibody [NBP1-28744]

Western Blot: CENPT Antibody [NBP1-28744]

Western Blot: CENPT Antibody [NBP1-28744] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CENP-T antibody NBP1-28744 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. CENP-T was also immunoprecipitated by rabbit anti-CENP-T antibody NBP1-28698, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

Applications for CENPT Antibody

Application
Recommended Usage

Immunoprecipitation

10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CENPT

Centromere protein T (CENP-T) is a component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENP-T is one of the basic inner kinetochore proteins with most further proteins binding downstream, suggesting a fundamental role of CENP-T in kinetochore function.

Alternate Names

C16orf56, CENP-Tchromosome 16 open reading frame 56, centromere protein T, FLJ13111, FLJ43376, ICEN22, Interphase centromere complex protein 22

Entrez Gene IDs

80152 (Human)

Gene Symbol

CENPT

UniProt

Q96BT3 (Human)

Additional CENPT Products

Product Documents for CENPT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CENPT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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