Centrin 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14909
Key Product Details
Species Reactivity
Validated:
Human, Bovine
Predicted:
Mouse (95%), Rat (95%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Centrin 1. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (84%), Chicken (84%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Centrin 1 Antibody
![Western Blot: Centrin 1 Antibody [NBP2-14909]](https://resources.bio-techne.com/images/products/Centrin-1-Antibody-Western-Blot-NBP2-14909-img0003.jpg "Western Blot: Centrin 1 Antibody [NBP2-14909]")
Western Blot: Centrin 1 Antibody [NBP2-14909]
Western Blot: Centrin 1 Antibody [NBP2-14909] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin 1 antibody diluted at a dilution of 1:1000.![Immunoprecipitation: Centrin 1 Antibody [NBP2-14909]](https://resources.bio-techne.com/images/products/Centrin-1-Antibody-Immunoprecipitation-NBP2-14909-img0006.jpg "Immunoprecipitation: Centrin 1 Antibody [NBP2-14909]")
Immunoprecipitation: Centrin 1 Antibody [NBP2-14909]
Immunoprecipitation: Centrin 1 Antibody [NBP2-14909] - Immunoprecipitation of Centrin-1 protein from 293T whole cell extracts using 5 ug of Centrin-1 antibody [N1C3] . Western blot analysis was performed using Centrin-1 antibody [N1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Immunoprecipitation: Centrin 1 Antibody [NBP2-14909] -
Immunoprecipitation: Centrin 1 Antibody [NBP2-14909] - A431 cells were fixed in ice-cold MeOH for 5 min.Green: Centrin 1 stained by Centrin 1 antibody [N1C3] diluted at 1:500.Blue: Hoechst 33342 staining.Applications for Centrin 1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry-Paraffin
Assay dependent
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Centrin 1
Alternate Names
calcium binding protein, caltractin, Caltractin isoform 2, CEN1centrin-1, centrin, EF-hand protein, 1, CETN, EF-hand protein
Gene Symbol
CETN1
UniProt
Additional Centrin 1 Products
Product Documents for Centrin 1 Antibody
Product Specific Notices for Centrin 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.