Centrin 3 Antibody (3E6)

Novus Biologicals, part of Bio-Techne | Catalog # H00001070-M01

Novus Biologicals, part of Bio-Techne
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H00001070-M01-50ug
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken, Zebrafish

Cited:

Human, Mouse, Fish - Danio rerio (Zebrafish)

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3E6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Centrin 3 Antibodies »

Product Specifications

Immunogen

CETN3 (AAH05383, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSLALRSELVVDKTKRKKRRELSEEQKQEIKDAFELFDTDKDEAIDYHELKVAMRALGFDVKKADVLKILKDYDREATGKITFEDFNEVVTDWILERDPH

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 25344692).

Specificity

CETN3 - centrin, EF-hand protein, 3 (CDC31 homolog, yeast)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Centrin 3 Antibody (3E6)

Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01]

Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01]

Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01] - Analysis of 293T cells using Centrin 3 antibody (3E6). Image from verified customer review.
Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01]

Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01]

Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01] - CETN3 monoclonal antibody (M01), clone 3E6 Analysis of CETN3 expression in Jurkat.
Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01]

Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01]

Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01] - Analysis of CETN3 expression in transfected 293T cell line by CETN3 monoclonal antibody (M01), clone 3E6.Lane 1: CETN3 transfected lysate(19.5 KDa).Lane 2: Non-transfected lysate.
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Applications for Centrin 3 Antibody (3E6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. ICC/IF usage reported in scientific literature (PMID: 23644468). Use in Immunoprecipitation reported in scientific literature (PMID: 26354417)

Reviewed Applications

Read 1 review rated 5 using H00001070-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Centrin 3

The protein encoded by this gene contains four EF-hand calcium binding domains, and is a member of the centrin protein family. Centrins are evolutionarily conserved proteins similar to the CDC31 protein of S. cerevisiae. Yeast CDC31 is located at the centrosome of interphase and mitotic cells, where it plays a fundamental role in centrosome duplication and separation. Multiple forms of the proteins similar to the yeast centrin have been identified in human and other mammalian cells, some of which have been shown to be associated with centrosome fractions. This protein appears to be one of the most abundant centrins associated with centrosome, which suggests a similar function to its yeast counterpart.

Alternate Names

CDC31 yeast homolog, CEN3centrin, EF-hand protein, 3 (CDC31 homolog, yeast), centrin, EF-hand protein, 3, centrin, EF-hand protein, 3 (CDC31 yeast homolog), centrin-3, EF-hand superfamily member, MGC12502, MGC138245

Entrez Gene IDs

1070 (Human)

Gene Symbol

CETN3

OMIM

602907 (Human)

UniProt

AAH05383 (Human)

Additional Centrin 3 Products

Product Documents for Centrin 3 Antibody (3E6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Centrin 3 Antibody (3E6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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