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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1800 and 1850 of human centrosomal protein using the numbering given in entry NP_115518.2 (GeneID 55125).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CEP192 Antibody

Western Blot: CEP192 Antibody [NBP1-28718]

Western Blot: CEP192 Antibody [NBP1-28718]

Western Blot: CEP192 Antibody [NBP1-28718] - Detection of Human CEP192 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CEP192 antibody NBP1-28718 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. CEP192 was also immunoprecipitated by rabbit anti-CEP192 antibody BL6868, which recognizes an upstream epitope. For blotting immunoprecipitated CEP192, the ReliaBLOT® Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for CEP192 Antibody

Application
Recommended Usage

Immunoprecipitation

10 ug/mg lysate

Western Blot

1:1000-1:5000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-12% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CEP192

Centrosomal protein of 192 kDa (CEP192) has been shown to be required for centrosomes maturation and spindle assembly. In this process, CEP192 functions as scaffolding for microtubule nucleation during mitosis.

Alternate Names

centrosomal protein 192kDa, centrosomal protein of 192 kDa, Cep192, FLJ10352, KIAA1569

Entrez Gene IDs

55125 (Human)

Gene Symbol

CEP192

UniProt

Additional CEP192 Products

Product Documents for CEP192 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CEP192 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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