CEP350 Antibody (CL3423)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59028

Novus Biologicals, part of Bio-Techne
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NBP2-59028
NBP2-59028-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3423

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CEP350 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:QVVQSQREVTEVLQEATCKIAAQQSETARLTTDAARQICEMAELTRTHISDAVVASGAPLAILYDHQRQHLPDFVKQLRTRTETDRKSPSVSLSQ

Epitope

LPDFVKQLRTRT

Reactivity Notes

Expected to cross react based on sequence identity: Mouse (81%), Rat (83%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CEP350 Antibody (CL3423)

Immunocytochemistry/ Immunofluorescence: CEP350 Antibody (CL3423) [NBP2-59028]

Immunocytochemistry/ Immunofluorescence: CEP350 Antibody (CL3423) [NBP2-59028]

Immunocytochemistry/Immunofluorescence: CEP350 Antibody (CL3423) [NBP2-59028] - Staining of U251 cells using the anti-CEP350 monoclonal antibody, showing specific staining of the centrosome in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CEP350 Antibody (CL3423) [NBP2-59028]

Immunohistochemistry-Paraffin: CEP350 Antibody (CL3423) [NBP2-59028]

Immunohistochemistry-Paraffin: CEP350 Antibody (CL3423) [NBP2-59028] - Staining of human placenta shows centrosome-like immunoreactivity in trophoblast cells.
Immunocytochemistry/ Immunofluorescence: CEP350 Antibody (CL3423) [NBP2-59028]

Immunocytochemistry/ Immunofluorescence: CEP350 Antibody (CL3423) [NBP2-59028]

Immunocytochemistry/Immunofluorescence: CEP350 Antibody (CL3423) [NBP2-59028] - Staining of A431 cells using the anti-CEP350 monoclonal antibody, showing specific staining of the centrosome in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
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Applications for CEP350 Antibody (CL3423)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEP350

CAP350 (Centrosome-Associated Protein 350) has been shown to localize to the centrosome as well as to dots in the pericentrosomal area. CAP350 functions to stabilize and anchor microtubules to the centrosome and participates in the maintenance of a pericentrosomal Golgi ribbon.

Alternate Names

CAP350GM133, centrosomal protein 350kDa, centrosome-associated protein 350, Centrosome-associated protein of 350 kDa, Cep350, FLJ38282, FLJ44058, KIAA0480centrosome associated protein 350

Gene Symbol

CEP350

Additional CEP350 Products

Product Documents for CEP350 Antibody (CL3423)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CEP350 Antibody (CL3423)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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