Ceruloplasmin Antibody (6C3K9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15868

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15868-100ul
NBP3-15868-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6C3K9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ceruloplasmin Antibodies »

Product Summary for Ceruloplasmin Antibody (6C3K9)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 366-572 of human Ceruloplasmin (NP_000087.1). IRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQGTTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAYPLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNADPVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

122 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ceruloplasmin Antibody (6C3K9)

Western Blot: Ceruloplasmin Antibody (6C3K9) [NBP3-15868]

Western Blot: Ceruloplasmin Antibody (6C3K9) [NBP3-15868]

Western Blot: Ceruloplasmin Antibody (6C3K9) [NBP3-15868] - Analysis of extracts of various cell lines, using Ceruloplasmin antibody (NBP3-15868) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry: Ceruloplasmin Antibody (6C3K9) [NBP3-15868]

Immunohistochemistry: Ceruloplasmin Antibody (6C3K9) [NBP3-15868]

Immunohistochemistry: Ceruloplasmin Antibody (6C3K9) [NBP3-15868] - Immunofluorescence analysis of mouse liver using Ceruloplasmin Rabbit mAb (NBP3-15868) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Ceruloplasmin Antibody (6C3K9) [NBP3-15868]

Immunohistochemistry-Paraffin: Ceruloplasmin Antibody (6C3K9) [NBP3-15868]

Immunohistochemistry-Paraffin: Ceruloplasmin Antibody (6C3K9) [NBP3-15868] - Rat liver using Ceruloplasmin Rabbit mAb (NBP3-15868) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Applications for Ceruloplasmin Antibody (6C3K9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

1:2000 - 1:6000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ceruloplasmin

The protein encoded by the Ceruloplasmin gene is a metalloprotein that binds most of the copper in plasma and is involved in theperoxidation of Fe(II)transferrin to Fe(III) transferrin. Mutations in this gene cause aceruloplasminemia, whichresults in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities.(provided by RefSeq)

Alternate Names

ceruloplasmin, ceruloplasmin (ferroxidase), CP-2, EC 1.16.3.1, Ferroxidase

Gene Symbol

CP

Additional Ceruloplasmin Products

Product Documents for Ceruloplasmin Antibody (6C3K9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ceruloplasmin Antibody (6C3K9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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