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CFAP300 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81213

Novus Biologicals, part of Bio-Techne
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NBP1-81213
NBP1-81213-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RLYDEDIVRDSGHIVKCLDSFCDPFLISDELRRVLLVEDSEKYEIFSQPDREEFLFCLFKHLCLGGALCQYEDVIS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CFAP300 Antibody

Western Blot: CFAP300 Antibody [NBP1-81213]

Western Blot: CFAP300 Antibody [NBP1-81213]

Western Blot: C11orf70 Antibody [NBP1-81213] - Analysis in control (vector only transfected HEK293T lysate) and C11orf70 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: CFAP300 Antibody [NBP1-81213]

Immunocytochemistry/ Immunofluorescence: CFAP300 Antibody [NBP1-81213]

Immunocytochemistry/Immunofluorescence: C11orf70 Antibody [NBP1-81213] - Staining of human cell line SiHa shows localization to centrosome.
Immunohistochemistry-Paraffin: CFAP300 Antibody [NBP1-81213]

Immunohistochemistry-Paraffin: CFAP300 Antibody [NBP1-81213]

Immunohistochemistry-Paraffin: C11orf70 Antibody [NBP1-81213] - Staining in human fallopian tube and prostate tissues using anti-C11orf70 antibody. Corresponding C11orf70 RNA-seq data are presented for the same tissues.

Applications for CFAP300 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CFAP300

Alternate Names

chromosome 11 open reading frame 70, hypothetical protein LOC85016, MGC13040

Entrez Gene IDs

85016 (Human)

Gene Symbol

C11ORF70

Additional CFAP300 Products

Product Documents for CFAP300 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CFAP300 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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