cGK1/PRKG1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87289

Novus Biologicals, part of Bio-Techne
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NBP1-87289
NBP1-87289-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all cGK1/PRKG1 Antibodies »

Product Summary for cGK1/PRKG1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LADVLEETHYENGEYIIRQGARGDTFFIISKGTVNVTREDSPSEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for cGK1/PRKG1 Antibody

Immunocytochemistry/ Immunofluorescence: cGK1/PRKG1 Antibody [NBP1-87289]

Immunocytochemistry/ Immunofluorescence: cGK1/PRKG1 Antibody [NBP1-87289]

Immunocytochemistry/Immunofluorescence: cGK1/PRKG1 Antibody [NBP1-87289] - Staining of human cell line HEK 293 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: cGK1/PRKG1 Antibody [NBP1-87289]

Immunohistochemistry-Paraffin: cGK1/PRKG1 Antibody [NBP1-87289]

Immunohistochemistry-Paraffin: cGK1/PRKG1 Antibody [NBP1-87289] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: cGK1/PRKG1 Antibody [NBP1-87289]

Immunohistochemistry-Paraffin: cGK1/PRKG1 Antibody [NBP1-87289]

Immunohistochemistry-Paraffin: cGK1/PRKG1 Antibody [NBP1-87289] - Staining of human lung shows moderate cytoplasmic and membranous positivity in pneumocytes.
View 2 more images

Applications for cGK1/PRKG1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: cGK1/PRKG1

cGK1/PRKG1, also known as cGMP-dependent protein kinase 1, has two isoforms that are produced by alternative splicing. Isoform Alpha (CGK1-alpha) is 671 amino acids long and 76 kDa. Isoform Beta (CGK1-beta) is 686 amino acids long and approximately 78 kDa. PRKG1 is a Serine/threonine protein kinase that is expressed in placenta and lung. cGK1/PRKG1 is an important player in the Nitric Oxide/cGMP Signaling Pathway; the binding of GMP activates PRKG1, which in turn phosphorylates Serine residues and Threonine residues on various cell proteins, including proteins that regulate calcium levels, smooth muscle contraction, platelet adhesion, circadian rhythm and more. Current research on PRKG1 is being conducted in relation to several diseases and disorders including cystic fibrosis, lymphoma, non-small cell lung carcinoma, Wiskott-Aldrich syndrome, Waterhouse-Friderichsen syndrome and myoglobinuria. PRKG1 has also been shown to have interactions with SMAD4, BMPR2, GKAP1, Natriuretic Peptide Receptor A and TFII-I in pathways such as Gap Junction signaling, eNOS signaling and EDNRB signaling.

Long Name

cGMP-dependent protein kinase 1

Alternate Names

cGK 1, cGK1, cGKI, EC 2.7.11.12, PRKG1, PRKG1B, PRKGR1A, PRKGR1B

Gene Symbol

PRKG1

Additional cGK1/PRKG1 Products

Product Documents for cGK1/PRKG1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for cGK1/PRKG1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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