ch-TOG Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06513

Novus Biologicals, part of Bio-Techne
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NBP1-06513
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Amphibian, Xenopus

Cited:

Amphibian

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all ch-TOG Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1982 and 2032 of human colonic and hepatic tumor over-expressed protein using the numbering given in entry NP_001008938.1 (GeneID 9793).

Reactivity Notes

Xenopus reactivity reported in scientific literature (PMID: 29851557). Amphibian reactivity reported in scientific literature (PMID:29851557).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ch-TOG Antibody

Western Blot: ch-TOG Antibody [NBP1-06513]

Western Blot: ch-TOG Antibody [NBP1-06513]

Western Blot: ch-TOG Antibody [NBP1-06513] - Western Blot and Immunoprecipitation: ch-TOG antibody [NBP1-06513]- Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ch-TOG antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ch-TOG was also immunoprecipitated by rabbit anti-ch-TOG antibodies NBP1-06511and NBP1-06512, which recognize upstream epitopes.

Applications for ch-TOG Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ch-TOG

Colonic and hepatic tumor over-expressed protein (ch-TOG) is a protein originally described as a gene over-expressed in human hepatomas and colonic tumors. It was found to be a homolog of the xenopus egg protein, XMAP214, a microtubule associated protein (MAP). The human ch-TOG protein has been shown to promote microtubule assembly and is a major component of microtubule asters. It is required for centrosome integrity, spindle pole organization and bipolar spindle assembly. Alternative names for ch-TOG include cytoskeleton-associated protein 5, CKAP5, TOG, MSPS, TOGp, and KIAA0097.

Alternate Names

CHTOG, ch-TOG, Colonic and hepatic tumor over-expressed gene protein, cytoskeleton associated protein 5, cytoskeleton-associated protein 5, EC 2.7.7.6, EC 3.1.1.29, KIAA0097FLJ35359, MSPS, TOG, TOGp

Entrez Gene IDs

9793 (Human); 75786 (Mouse)

Gene Symbol

CKAP5

UniProt

Q14008 (Human)

Additional ch-TOG Products

Product Documents for ch-TOG Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ch-TOG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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