ch-TOG Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85624

Novus Biologicals, part of Bio-Techne
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NBP1-85624
NBP1-85624-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ch-TOG Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MSSKLNQARSMSGHPEAAQMVRREFQLDLDEIENDNGTVRCEMPELVQHKLDDIFEPVLIPEPKIRAVSPHFDDMHSNTASTINFI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ch-TOG Antibody

Immunocytochemistry/ Immunofluorescence: ch-TOG Antibody [NBP1-85624]

Immunocytochemistry/ Immunofluorescence: ch-TOG Antibody [NBP1-85624]

Immunocytochemistry/Immunofluorescence: ch-TOG Antibody [NBP1-85624] - Staining of human cell line U-2 OS shows localization to nucleoli, plasma membrane & centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ch-TOG Antibody [NBP1-85624]

Immunohistochemistry-Paraffin: ch-TOG Antibody [NBP1-85624]

Immunohistochemistry-Paraffin: ch-TOG Antibody [NBP1-85624] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: ch-TOG Antibody [NBP1-85624]

Immunohistochemistry-Paraffin: ch-TOG Antibody [NBP1-85624]

Immunohistochemistry-Paraffin: ch-TOG Antibody [NBP1-85624] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
View 2 more images

Applications for ch-TOG Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ch-TOG

ch-TOG, also known as CKAP-5, is a microtubule-associated protein that is involved in microtubule dynamics required for mitotic spindle formation. Specifically, chTOG protects kinetochore microtubules from depolymerization and plays an important role in centrosomal microtubule assembly. ch-TOG is typically overexpressed in tumor cells, may therefore be associated with cancer development.

ch-TOG antibodies are useful tools for cytosleletal research and for some cancer studies.

Alternate Names

CHTOG, ch-TOG, Colonic and hepatic tumor over-expressed gene protein, cytoskeleton associated protein 5, cytoskeleton-associated protein 5, EC 2.7.7.6, EC 3.1.1.29, KIAA0097FLJ35359, MSPS, TOG, TOGp

Gene Symbol

CKAP5

Additional ch-TOG Products

Product Documents for ch-TOG Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ch-TOG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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