CH25H Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-83971
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen is a synthetic peptide directed towards the C-terminal region of Human CH25H. Peptide sequence: VTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHH The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for CH25H Antibody
Western Blot: CH25H Antibody [NBP2-83971]
Western Blot: CH25H Antibody [NBP2-83971] - WB Suggested Anti-CH25H Antibody. Titration: 1.0 ug/ml. Positive Control: Fetal BrainWestern Blot: CH25H Antibody [NBP2-83971]
Western Blot: CH25H Antibody [NBP2-83971] - Host: Rabbit. Target Name: CH25H. Sample Tissue: Human MCF7 Whole Cell. Antibody Dilution: 1ug/mlWestern Blot: CH25H Antibody [NBP2-83971] -
Western Blot: CH25H Antibody [NBP2-83971] - Cholesterol Hydroxylases are Upregulated by IL-1 beta. IL-1 beta induces the expression of cholesterol hydroxylase CH25H in FLS. CH25H was measured by western blot and quantified using imageJ. FLS were treated with 1 ng/mL IL-1 beta or 0.1 uM T0901317 for 24, 48, and 72 h. Data for biological replicates represent fold-change versus corresponding controls (= 1, indicated by broken horizontal lines) and are shown as dot plots, with lines inside showing the mean +/- SD (n = 8). The y-axis represents the log of variable fold changes. Black circles show data from IL-1 beta-treated FLS relative to untreated controls, whereas blue squares represent data from T0901317 relative to vehicle controls. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35269552/) licensed under a CC-BY license.Applications for CH25H Antibody
Application
Recommended Usage
Western Blot
1.0 ug/ml
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CH25H
Alternate Names
C25H, cholesterol 25-hydroxylase, Cholesterol 25-monooxygenase, EC 1.14.99.38, h25OH
Gene Symbol
CH25H
Additional CH25H Products
Product Specific Notices for CH25H Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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