Skip to main content

CH25H Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-83971

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-83971-0.1ml

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

0.5 mg/ml

Product Specifications

Immunogen

The immunogen is a synthetic peptide directed towards the C-terminal region of Human CH25H. Peptide sequence: VTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHH The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for CH25H Antibody

Western Blot: CH25H Antibody [NBP2-83971]

Western Blot: CH25H Antibody [NBP2-83971]

Western Blot: CH25H Antibody [NBP2-83971] - WB Suggested Anti-CH25H Antibody. Titration: 1.0 ug/ml. Positive Control: Fetal Brain
Western Blot: CH25H Antibody [NBP2-83971]

Western Blot: CH25H Antibody [NBP2-83971]

Western Blot: CH25H Antibody [NBP2-83971] - Host: Rabbit. Target Name: CH25H. Sample Tissue: Human MCF7 Whole Cell. Antibody Dilution: 1ug/ml
CH25H Antibody

Western Blot: CH25H Antibody [NBP2-83971] -

Western Blot: CH25H Antibody [NBP2-83971] - Cholesterol Hydroxylases are Upregulated by IL-1 beta. IL-1 beta induces the expression of cholesterol hydroxylase CH25H in FLS. CH25H was measured by western blot and quantified using imageJ. FLS were treated with 1 ng/mL IL-1 beta or 0.1 uM T0901317 for 24, 48, and 72 h. Data for biological replicates represent fold-change versus corresponding controls (= 1, indicated by broken horizontal lines) and are shown as dot plots, with lines inside showing the mean +/- SD (n = 8). The y-axis represents the log of variable fold changes. Black circles show data from IL-1 beta-treated FLS relative to untreated controls, whereas blue squares represent data from T0901317 relative to vehicle controls. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35269552/) licensed under a CC-BY license.

Applications for CH25H Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CH25H

CH25H that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq]

Alternate Names

C25H, cholesterol 25-hydroxylase, Cholesterol 25-monooxygenase, EC 1.14.99.38, h25OH

Gene Symbol

CH25H

Additional CH25H Products

Product Documents for CH25H Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CH25H Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...