CH25H Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05283
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
CH25H Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of human CH25H conjugated to Keyhole Limpet Hemocyanin (KLH).
(Uniprot: O95992)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence and 92% homology to pig, 91% homology to rat
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human CH25H. This product was affinity purified from monospecific antiserum by immunoaffinity purification
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human CH25H. This product was affinity purified from monospecific antiserum by immunoaffinity purification
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for CH25H Antibody
Western Blot: CH25H Antibody [NBP3-05283]
Western Blot: CH25H Antibody [NBP3-05283] - Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: CH25H overexpressed HEK293T Lysate [10ug]. Lane 3: HeLa whole cell Lysate [10ug]. Primary Antibody: CH25H Antibody at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:70,000 for 30min at RT. Block: Universal BlockOut Buffer. Predicted MW: ~31.7kDa. Observed MW: ~35kDa. Exposure: 3 seconds.Immunohistochemistry: CH25H Antibody [NBP3-05283]
Immunohistochemistry: CH25H Antibody [NBP3-05283] - Tissue: Human Kidney. Fixative: None. Antigen Retrieval: HIER using Citrate Buffer for 20 minutes. Primary Antibody: CH25H Antibody at 1:100 for 30 min at RT. Secondary Antibody: Anti-Rabbit Poly-HRP IgG Ready-to-Use for 8 minutes at RT. Counterstain: Hematoxylin. Substrate: DAB. Staining: (A) Negative control. (B) 20X. (C) 40X. Results: Shows strong cytoplasmic staining of renal tubular epitheliumCH25H Antibody
Immunohistochemistry of CH25H Antibody. Tissue: Human Kidney. Fixative: None. Antigen Retrieval: HIER using Citrate Buffer for 20 minutes. Primary Antibody: Anti-CH25H at 1:100 for 30 min at RT. Secondary Antibody: Anti-Rabbit Poly-HRP IgG Ready-to-Use for 8 minutes at RT. Counterstain: Hematoxylin. Substrate: DAB. Staining: (A) Negative control. (B) 20X. (C) 40X. Results: Shows strong cytoplasmic staining of renal tubular epithelium.Applications for CH25H Antibody
Application
Recommended Usage
ELISA
1:10000-1:50000
Immunohistochemistry
1:100
Western Blot
1:1000
Application Notes
This product has been tested in ELISA, WB, and IHC. Expect a band at ~31.7kDa in western blot using appropriate tissues or lysates. Positive control used: Human Kidney Tissues in Immunohistochemistry.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: CH25H
Alternate Names
C25H, cholesterol 25-hydroxylase, Cholesterol 25-monooxygenase, EC 1.14.99.38, h25OH
Gene Symbol
CH25H
UniProt
Additional CH25H Products
Product Documents for CH25H Antibody
Product Specific Notices for CH25H Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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