CHCHD2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-94106

Novus Biologicals, part of Bio-Techne
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NBP1-94106
NBP1-94106-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CHCHD2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YQQHQGTQPAQQQQPCLYEIKQFLECAQNQGDIKLCEGFNEVLKQCRLANR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHCHD2 Antibody

Western Blot: CHCHD2 Antibody [NBP1-94106]

Western Blot: CHCHD2 Antibody [NBP1-94106]

Western Blot: CHCHD2 Antibody [NBP1-94106] - Analysis in human cell lines A-431 and HeLa using anti-CHCHD2 antibody. Corresponding CHCHD2 RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.
Immunocytochemistry/ Immunofluorescence: CHCHD2 Antibody [NBP1-94106]

Immunocytochemistry/ Immunofluorescence: CHCHD2 Antibody [NBP1-94106]

Immunocytochemistry/Immunofluorescence: CHCHD2 Antibody [NBP1-94106] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CHCHD2 Antibody [NBP1-94106]

Immunohistochemistry-Paraffin: CHCHD2 Antibody [NBP1-94106]

Immunohistochemistry-Paraffin: CHCHD2 Antibody [NBP1-94106] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
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Applications for CHCHD2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHCHD2

Alternate Names

16.7kD protein, AAG10, coiled-coil-helix-coiled-coil-helix domain containing 2, coiled-coil-helix-coiled-coil-helix domain-containing protein 2, mitochondrial, HCV NS2 trans-regulated protein

Entrez Gene IDs

51142 (Human)

Gene Symbol

CHCHD2

UniProt

Q9Y6H1 (Human)

Additional CHCHD2 Products

Product Documents for CHCHD2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CHCHD2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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