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CHCHD2 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92335

Novus Biologicals, part of Bio-Techne
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NBP2-92335-0.02ml
NBP2-92335-0.1ml

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for CHCHD2 Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 75-145 of human CHCHD2 (NP_057223.1). TLGHAITGGFSGGSNAEPARPDITYQEPQGTQPAQQQQPCLYEIKQFLECAQNQGDIKLCEGFNEVLKQCR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CHCHD2 Antibody - Azide and BSA Free

Western Blot: CHCHD2 AntibodyAzide and BSA Free [NBP2-92335]

Western Blot: CHCHD2 AntibodyAzide and BSA Free [NBP2-92335]

Western Blot: CHCHD2 Antibody [NBP2-92335] - Western blot analysis of extracts from normal (control) and CHCHD2 knockout (KO) 293T cells, using CHCHD2 antibody (NBP2-92335) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: CHCHD2 Antibody - Azide and BSA Free [NBP2-92335]

Immunocytochemistry/ Immunofluorescence: CHCHD2 Antibody - Azide and BSA Free [NBP2-92335]

Immunocytochemistry/Immunofluorescence: CHCHD2 Antibody [NBP2-92335] - Analysis of C6 cells using CHCHD2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: CHCHD2 Antibody - Azide and BSA Free [NBP2-92335]

Immunohistochemistry-Paraffin: CHCHD2 Antibody - Azide and BSA Free [NBP2-92335]

Immunohistochemistry-Paraffin: CHCHD2 Antibody [NBP2-92335] - Immunohistochemistry of paraffin-embedded Rat ovary using [KO Validated] CHCHD2 Rabbit pAb (NBP2-92335) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for CHCHD2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CHCHD2

Alternate Names

16.7kD protein, AAG10, coiled-coil-helix-coiled-coil-helix domain containing 2, coiled-coil-helix-coiled-coil-helix domain-containing protein 2, mitochondrial, HCV NS2 trans-regulated protein

Gene Symbol

CHCHD2

Additional CHCHD2 Products

Product Documents for CHCHD2 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CHCHD2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov