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CHCHD3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21606

Novus Biologicals, part of Bio-Techne
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NBP2-21606

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (90%), Porcine (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CHCHD3. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (83%).

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CHCHD3 Antibody

Western Blot: CHCHD3 Antibody [NBP2-21606]

Western Blot: CHCHD3 Antibody [NBP2-21606]

Western Blot: CHCHD3 Antibody [NBP2-21606] - Wild-type (WT) and CHCHD3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CHCHD3 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: CHCHD3 Antibody [NBP2-21606]

Immunohistochemistry-Paraffin: CHCHD3 Antibody [NBP2-21606]

Immunohistochemistry-Paraffin: CHCHD3 Antibody [NBP2-21606] - CHCHD3 antibody detects CHCHD3 protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CHCHD3 antibody diluted at 1:500.
Western Blot: CHCHD3 Antibody [NBP2-21606]

Western Blot: CHCHD3 Antibody [NBP2-21606]

Western Blot: CHCHD3 Antibody [NBP2-21606] - Sample (30 ug of whole cell lysate) A: 293T B: A431 12% SDS PAGE gel, diluted at 1:5000.

Applications for CHCHD3 Antibody

Application
Recommended Usage

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CHCHD3

Alternate Names

coiled-coil-helix-coiled-coil-helix domain containing 3, FLJ20420, mitochondrial

Gene Symbol

CHCHD3

UniProt

Additional CHCHD3 Products

Product Documents for CHCHD3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CHCHD3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.