CHD1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-60411
![Knockdown Validated: CHD1 Antibody [NB100-60411]](https://resources.bio-techne.com/images/products/CHD1-Antibody-Knockdown-Validated-NB100-60411-img0008.jpg "Knockdown Validated: CHD1 Antibody [NB100-60411]")
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Chicken (100%). Backed by our 100% Guarantee.
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, Immunoprecipitation, Knockdown Validated, Knockout Validated, Western Blot
Cited:
Chemotaxis, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1660 and 1710 of human chromodomain helicase DNA binding protein 1 using the numbering given in entry NP_001261.2 (GeneID 1105).
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 20161795).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CHD1 Antibody
![Knockout Validated: CHD1 Antibody [NB100-60411]](https://resources.bio-techne.com/images/products/CHD1-Antibody-Knockout-Validated-NB100-60411-img0007.jpg "Knockout Validated: CHD1 Antibody [NB100-60411]")
Knockout Validated: CHD1 Antibody [NB100-60411]
Knockout Validated: CHD1 Antibody [NB100-60411] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and CHD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human CHD1 Polyclonal Antibody (Catalog # NB100-60411) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CHD1 at approximately 260 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.![Western Blot: CHD1 Antibody [NB100-60411]](https://resources.bio-techne.com/images/products/CHD1-Antibody-Western-Blot-NB100-60411-img0005.jpg "Western Blot: CHD1 Antibody [NB100-60411]")
Western Blot: CHD1 Antibody [NB100-60411]
Western Blot: CHD1 Antibody [NB100-60411] - Detection of Human CHD1 on HeLa whole cell lysate using NB100-60411. CHD1 was also immunoprecipitated by rabbit anti-CHD1 antibody NB100-60410.
Chromatin Immunoprecipitation Sequencing: CHD1 Antibody [NB100-60411] - Localization of CHD1 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CHD1 antibody NB100-60411 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CHD1 binding within a 3.5 Mb region of the human X chromosome as detected using anti-CHD1 antibody NB100-60411. ChIP-seq validation performed by Diogenode, Denville, NJ.
Applications for CHD1 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1-5 ug/mg lysate
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
WB in human was reported in the following publication: PMID: 21880597
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CHD1
Long Name
Chromodomain Helicase DNA Binding Protein 1
Alternate Names
ATP-dependent helicase CHD1, CHD-1, chromodomain helicase DNA binding protein 1, chromodomain-helicase-DNA-binding protein 1, DKFZp686E2337, EC 3.6.1, EC 3.6.4.12
Entrez Gene IDs
1105 (Human)
Gene Symbol
CHD1
UniProt
Additional CHD1 Products
Product Documents for CHD1 Antibody
Product Specific Notices for CHD1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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