CHD3 Antibody (8Z6L6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16632

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16632-100ul
NBP3-16632-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8Z6L6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CHD3 Antibodies »

Product Summary for CHD3 Antibody (8Z6L6)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CHD3 (Q12873). MKAADTVILWARSKNDQLRISFPPGLCWGDRMPDKDDIRLLPSALGVKKRKRGPKKQKENKPGKPRKRKKRDSEEEFGSERDEYREKSESGGSEYGTGPG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHD3 Antibody (8Z6L6)

Western Blot: CHD3 Antibody (8Z6L6) [NBP3-16632]

Western Blot: CHD3 Antibody (8Z6L6) [NBP3-16632]

Western Blot: CHD3 Antibody (8Z6L6) [NBP3-16632] - Western blot analysis of extracts of Mouse brain, using CHD3 Rabbit mAb (NBP3-16632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: CHD3 Antibody (8Z6L6) [NBP3-16632]

Immunocytochemistry/ Immunofluorescence: CHD3 Antibody (8Z6L6) [NBP3-16632]

Immunocytochemistry/Immunofluorescence: CHD3 Antibody (8Z6L6) [NBP3-16632] - Immunofluorescence analysis of NIH-3T3 cells using CHD3 Rabbit mAb (NBP3-16632) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: CHD3 Antibody (8Z6L6) [NBP3-16632]

Immunohistochemistry-Paraffin: CHD3 Antibody (8Z6L6) [NBP3-16632]

Immunohistochemistry-Paraffin: CHD3 Antibody (8Z6L6) [NBP3-16632] - Immunohistochemistry of paraffin-embedded mouse brain using CHD3 Rabbit mAb (NBP3-16632) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for CHD3 Antibody (8Z6L6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CHD3

CHD3 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD3 is a key component of the NuRD (nucleosomal remodeling) complex and directly interacts with histone deacetylases 1 and 2, ATR, and TRIM 27. Studies in several species suggest a role for CHD3 in the repression of developmental genes.

Alternate Names

ATP-dependent helicase CHD3, CHD-3, chromodomain helicase DNA binding protein 3, chromodomain-helicase-DNA-binding protein 3, EC 3.6.1, EC 3.6.4.12, hZFH, Mi-2 autoantigen 240 kDa protein, Mi-2a, Mi2-ALPHA, ZFH, Zinc finger helicase, zinc-finger helicase (Snf2-like)

Gene Symbol

CHD3

Additional CHD3 Products

Product Documents for CHD3 Antibody (8Z6L6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CHD3 Antibody (8Z6L6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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