CHD3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13040

Novus Biologicals, part of Bio-Techne
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NBP3-13040
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CHD3 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CHD3. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for CHD3 Antibody

Western Blot: CHD3 Antibody [NBP3-13040]

Western Blot: CHD3 Antibody [NBP3-13040]

Western Blot: CHD3 Antibody [NBP3-13040] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD3 antibody (NBP3-13040) diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: CHD3 Antibody [NBP3-13040]

Immunocytochemistry/ Immunofluorescence: CHD3 Antibody [NBP3-13040]

Immunocytochemistry/Immunofluorescence: CHD3 Antibody [NBP3-13040] - CHD3 antibody detects CHD3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CHD3 protein stained by CHD3 antibody (NBP3-13040) diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: CHD3 Antibody [NBP3-13040]

Immunohistochemistry-Paraffin: CHD3 Antibody [NBP3-13040]

Immunohistochemistry-Paraffin: CHD3 Antibody [NBP3-13040] - CHD3 antibody detects CHD3 protein at cytoplasm and nucleus in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CHD3 antibody (NBP3-13040) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for CHD3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHD3

CHD3 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD3 is a key component of the NuRD (nucleosomal remodeling) complex and directly interacts with histone deacetylases 1 and 2, ATR, and TRIM 27. Studies in several species suggest a role for CHD3 in the repression of developmental genes.

Alternate Names

ATP-dependent helicase CHD3, CHD-3, chromodomain helicase DNA binding protein 3, chromodomain-helicase-DNA-binding protein 3, EC 3.6.1, EC 3.6.4.12, hZFH, Mi-2 autoantigen 240 kDa protein, Mi-2a, Mi2-ALPHA, ZFH, Zinc finger helicase, zinc-finger helicase (Snf2-like)

Gene Symbol

CHD3

Additional CHD3 Products

Product Documents for CHD3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CHD3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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