CHD5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06267

Novus Biologicals, part of Bio-Techne
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NBP3-06267-100ul
NBP3-06267-50ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CHD5 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human CHD5 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CHD5 Antibody

Immunocytochemistry/ Immunofluorescence: CHD5 Antibody [NBP3-06267]

Immunocytochemistry/ Immunofluorescence: CHD5 Antibody [NBP3-06267]

Immunocytochemistry/Immunofluorescence: CHD5 Antibody [NBP3-06267] - Immunofluorescence staining of CHD5 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CHD5 polyclonal antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.
Immunohistochemistry-Paraffin: CHD5 Antibody [NBP3-06267]

Immunohistochemistry-Paraffin: CHD5 Antibody [NBP3-06267]

Immunohistochemistry-Paraffin: CHD5 Antibody [NBP3-06267] - Immunochemical staining of human CHD5 in cynomolgus cerebellum with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

Applications for CHD5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHD5

Changes in gene expression are associated with changes in chromatin structure, which can result from modification of histones by acetylation and methylation. In addition, SWI/SNF proteins can effect chromatin structure and gene expression by causing ATP-dependent disruption of the nucleosome and by enhancing binding of transcription factors. CHD5 belongs to a group of SWI/SNF proteins called CHD proteins, which contain a SWI/SNF-like helicase/ATPase domain, as well as a DNA-binding domain and a chromodomain that directly modifies chromatin structure (Thompson et al., 2003 (PubMed 12592387)).(supplied by OMIM)

Alternate Names

ATP-dependent helicase CHD5, CHD-5, chromodomain helicase DNA binding protein 5, chromodomain-helicase-DNA-binding protein 5, DKFZp434N231, EC 3.6.1, EC 3.6.4.12, KIAA0444

Gene Symbol

CHD5

Additional CHD5 Products

Product Documents for CHD5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CHD5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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