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ChemR23/CMKLR1 Antibody (BZ194) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80667

Novus Biologicals, part of Bio-Techne
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NBP2-80667
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # BZ194

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Summary for ChemR23/CMKLR1 Antibody (BZ194) - Azide and BSA Free

Immunogen

The immunogen for this antibody was CMKLR1.

Localization

Multi-pass membrane protein with cytoplasmic and extra-cellular domains

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for ChemR23/CMKLR1 Antibody (BZ194) - Azide and BSA Free

Immunohistochemistry-Paraffin: ChemR23/CMKLR1 Antibody (BZ194) - Azide and BSA Free [NBP2-80667]

Immunohistochemistry-Paraffin: ChemR23/CMKLR1 Antibody (BZ194) - Azide and BSA Free [NBP2-80667]

Immunohistochemistry-Paraffin: ChemR23/CMKLR1 Antibody (BZ194) - Azide and BSA Free [NBP2-80667] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of mouse liver using ChemR23/CMKLR1 antibody (clone BZ194) at 1:200 dilution. The antibody generated an expected membrane-cytoplasmic staining in the hepatocytes. Image from the standard
Flow Cytometry: ChemR23/CMKLR1 Antibody (BZ194) - Azide and BSA Free [NBP2-80667]

Flow Cytometry: ChemR23/CMKLR1 Antibody (BZ194) - Azide and BSA Free [NBP2-80667]

Flow Cytometry: ChemR23/CMKLR1 Antibody (BZ194) - Azide and BSA Free [NBP2-80667] - Staining of resident mouse peritoneal macrophages. Image from the standard format of this antibody.

Applications for ChemR23/CMKLR1 Antibody (BZ194) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:100 - 1:200
Application Notes
For FLOW validation, we tested this antibody on resident mouse peritoneal macrophages and found that it can be used at less than or equal to 0.5 ug per test (cell number should be determined empirically but can range from 10^5 to 10^8cells/test). In IHC-P, we tested it on mouse liver using routine protocol with heat induced antigen retrieval employing 10mM sodium citrate buffer pH 6
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ChemR23

The 194.7.4 (BZ194)monoclonal antibody reacts to mouse Chemoattractant-like receptor 1 (CMKLR1), also known as ChemR23 and DEZ. CMKLR1 is the receptor for chemerin, a potent chemoattractant. The BZ194 antibody was generated against the amino terminal residues of mouse CMKLR1. In the human CMKLR is found on pDC while in the mouse expression is confined to resident peritoneal macrophages with highest expression on Cd11blo macrophages. Expression is absent on in vitro cultured bone marrow DCs (except on early DCs as defined by CD11c+MHCIIint), and on freshly isolated CD11c+ cells from lymph node, spleen and bone marrow. Mouse CMKLR is thought to play a role in macrophage function as a result of injury.

Long Name

Chemokine-like Receptor 1/Orphan G Protein-coupled Receptor ChemR23

Alternate Names

cmklr1, Dez, Gpcr27

Gene Symbol

CMKLR1

Additional ChemR23 Products

Product Documents for ChemR23/CMKLR1 Antibody (BZ194) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ChemR23/CMKLR1 Antibody (BZ194) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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