ChemR23/CMKLR1 Antibody (BZ194) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11985CL1
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
CoraFluor 1
Antibody Source
Recombinant Monoclonal Rat IgG2a Kappa Clone # BZ194
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ChemR23/CMKLR1 Antibody (BZ194) [CoraFluor™ 1]
Immunogen
This antibody was raised by immunising Wistar Furth rats with the mChemR23/CMKLR1 peptide/KLH conjugate. The immunizing amino-terminal mChemR23/CMKLR1 peptide with the sequence NH2-DSGIYDDEYSDGFGYFVDLEEASPWC-COOH, corresponding to residues residues 8-32 of mChemR23/CMKLR1, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).
Specificity
This antibody is specific for murine serpentine receptor mChemR23/CMKLR1 (aka ChemR23, DEZ). mChemR23/CMKLR1 is a novel protein possessing high homology with members of the chemoattractant receptor family, and binds the chemoattractant chemerin. Selective mChemR23/CMKLR1 expression has been demonstrated by mouse macrophages ex vivo, and by dendritic cell precursors cultured in vitro.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for ChemR23/CMKLR1 Antibody (BZ194) [CoraFluor™ 1]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: ChemR23
Long Name
Chemokine-like Receptor 1/Orphan G Protein-coupled Receptor ChemR23
Alternate Names
cmklr1, Dez, Gpcr27
Gene Symbol
CMKLR1
Additional ChemR23 Products
Product Documents for ChemR23/CMKLR1 Antibody (BZ194) [CoraFluor™ 1]
Product Specific Notices for ChemR23/CMKLR1 Antibody (BZ194) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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