Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CHES1 Antibodies »

Product Summary for CHES1 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 440 and 490 of human Forkhead Box N3 using the numbering given in entry NP_001078940.1 (GeneID 1112).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHES1 Antibody

Immunoprecipitation: CHES1 Antibody [NBP2-04051]

Immunoprecipitation: CHES1 Antibody [NBP2-04051]

Immunoprecipitation: CHES1 Antibody [NBP2-04051] - Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FOXN3 antibody NBP2-04051 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. FOXN3 was also immunoprecipitated by rabbit anti-FOXN3 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for CHES1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CHES1

The CHES1 gene is a member of the forkhead/winged helix transcription factor family. Checkpoints are eukaryotic DNAdamage-inducible cell cycle arrests at G1 and G2. Checkpoint suppressor 1 suppresses multiple yeast checkpointmutations including mec1, rad9, rad

Alternate Names

C14orf116, Checkpoint suppressor 1PRO1635, CHES1forkhead box protein N3, chromosome 14 open reading frame 116, forkhead box N3

Gene Symbol

FOXN3

UniProt

NP_001078940.1 (Human)

Additional CHES1 Products

Product Documents for CHES1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CHES1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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