CHFR Antibody (2B11)

Novus Biologicals, part of Bio-Techne | Catalog # H00055743-M08

Novus Biologicals, part of Bio-Techne
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H00055743-M08
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2B11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CHFR Antibodies »

Product Specifications

Immunogen

CHFR (AAH12072.1, 136 a.a. ~ 194 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TSGAGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSC

Specificity

CHFR - checkpoint with forkhead and ring finger domains (2B11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CHFR Antibody (2B11)

Western Blot: CHFR Antibody (2B11) [H00055743-M08]

Western Blot: CHFR Antibody (2B11) [H00055743-M08]

Western Blot: CHFR Antibody (2B11) [H00055743-M08] - Analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody (M08), clone 2B11. Lane 1: CHFR transfected lysatE (72.1 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: CHFR Antibody (2B11) [H00055743-M08] - Detection limit for recombinant GST tagged CHFR is 0.03 ng/ml as a capture antibody.

Applications for CHFR Antibody (2B11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CHFR

E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress

Alternate Names

checkpoint with forkhead and ring finger domains, Checkpoint with forkhead and RING finger domains protein, E3 ubiquitin-protein ligase CHFR, EC 6.3.2.-, FLJ10796, FLJ33629, RING finger protein 196, RNF196RNF116

Entrez Gene IDs

55743 (Human)

Gene Symbol

CHFR

UniProt

AAH12072 (Human)

Additional CHFR Products

Product Documents for CHFR Antibody (2B11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CHFR Antibody (2B11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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