CHFR Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1166

Novus Biologicals, part of Bio-Techne
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NB100-1166
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all CHFR Antibodies »

Product Specifications

Immunogen

Peptide with sequence KFNHICEQTRFKN corresponding to C-Terminus according to NP_001154817.1, NP_001154818.1, NP_060693.2, NP_001154819.1.

Specificity

This antibody is expected to recognise all reported isoforms.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CHFR Antibody

Immunohistochemistry-Paraffin: CHFR Antibody [NB100-1166]

Immunohistochemistry-Paraffin: CHFR Antibody [NB100-1166]

Immunohistochemistry-Paraffin: CHFR Antibody [NB100-1166] - IHC analysis of CHFR in paraffin-embedded Human Brain (Cerebellum) using this antibody at 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for CHFR Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:64000
Application Notes
WB: Preliminary experiments gave an approx. 80 kDa band in human brain lysates at 0.5 ug/ml, this band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in scientific literature for the band we observe given the predicted MW of approx. 72 kDa according to NP_060693. IHC-P: Human brain (cerebellum) shows stained Purkinje cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CHFR

A number of human cancers are sensitive to mitotic stress, implying checkpoint defects. Many proteins that contain forkhead-associated (FHA) domains are cell cycle checkpoints and CHFR is one such protein. It has a FHA and ring finger domain within its N terminus. Within its C terminus, CHFR contains a cysteine-rich region that did not display significant similarity to any protein in the GenBank database, but is highly conserved between humans and mice. It has been concluded that CHFR defines a checkpoint that delays entry into metaphase in response to mitotic stress. It has been found CpG methylation-dependent silencing of CHFR expression occurs in 45% of cancer cell lines, 40% of primary colorectal cancers, 53% of colorectal adenomas, and 30% of primary head and neck cancers.

Alternate Names

checkpoint with forkhead and ring finger domains, Checkpoint with forkhead and RING finger domains protein, E3 ubiquitin-protein ligase CHFR, EC 6.3.2.-, FLJ10796, FLJ33629, RING finger protein 196, RNF196RNF116

Entrez Gene IDs

55743 (Human); 231600 (Mouse); 288734 (Rat)

Gene Symbol

CHFR

UniProt

NP_060693. (Human)

Additional CHFR Products

Product Documents for CHFR Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CHFR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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