Chicken Caronte Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1051
Key Product Details
Species Reactivity
Chicken
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 156808
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant chicken Caronte
Asp16-Asn272
Accession # NP_990154
Asp16-Asn272
Accession # NP_990154
Specificity
Detects chicken Caronte in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Cerberus, rhDAN, or recombinant mouse Gremlin is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Chicken Caronte Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Chicken Caronte Fc Chimera (Catalog # 1051-CA)
Sample: Recombinant Chicken Caronte Fc Chimera (Catalog # 1051-CA)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caronte
Chicken Caronte, the ortholog of mammalian Cerberus, is a member of the Cerberus/DAN family of secreted glycoproteins. There are at least 5 Dan Domain (DAND) family members including DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), Cerberus (DAND4), and Coco (DAND5). Caronte is expressed in the left paraxial mesoderm and is the molecule responsible for transducing left-sided positional information from the node to the periphery of the lateral plate mesoderm (LPM) during chicken embryonic development. Caronte functions as a BMP antagonist, inducing Nodal and downstream genes in the LPM by interfering with the repressive activity of bilaterally-produced BMPs. In addition, antagonism of BMPs by Caronte induces the expression of the Lefty gene in the midline. Caronte binds both BMP-4 and BMP-7 in immunoprecipitation experiments. Recombinant chicken Caronte preparations from R&D Systems have been shown to bind BMP-4 in a functional ELISA and induce heart reversals in Xenopus embryos.
References
- Pearce, J. et al. (1999) Dev. Biol. 209:98.
- Yokouchi, Y. et al. (1999) Cell. 98:573.
- King, T. and N. Brown (1999) Nature 401:222.
- Ahu, L. et al. (1999) Curr. Biol. 9:931.
- Rodriguez-Esteban, C. et al. (1999) Nature 401:243.
Alternate Names
Chicken Caronte
Entrez Gene IDs
395623 (Chicken)
Gene Symbol
CER1
UniProt
Additional Caronte Products
Product Documents for Chicken Caronte Antibody
Product Specific Notices for Chicken Caronte Antibody
For research use only
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