Chicken RGM-A Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2010

R&D Systems, part of Bio-Techne
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AF2010
AF2010-SP
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Conjugate
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Biotin
BAF2010
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Key Product Details

Species Reactivity

Validated:

Chicken

Cited:

Avian - Chicken

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all RGM-A Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant chicken RGM-A
Trp30-Asn404 (Asn404Asp)
Accession # Q8JG54

Specificity

Detects chicken RGM-A in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant human RGM and recombinant mouse RGM.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Chicken RGM-A Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Chicken Repulsive Guidance Molecule A/RGM-A

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RGM-A

Chicken RGM is the founding member of the RGM family of glycosylphosphatidylinositol (GPI)-anchored axon guidance proteins. It is a disulfide-linked dimer of the N-terminal RGD-containing segment (aa 30‑149) and the C-terminal segment (aa 150‑432). RGM-A binds with high affinity to neogenin. Chicken RGM-A (aa 30‑401) shares 88% and 87% aa sequence identity with its human and mouse homologs, respectively.

Long Name

Repulsive Guidance Molecule A

Alternate Names

RGMA

Entrez Gene IDs

56963 (Human); 244058 (Mouse)

Gene Symbol

RGMA

UniProt

Q8JG54

Additional RGM-A Products

Product Documents for Chicken RGM-A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Chicken RGM-A Antibody

For research use only

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