Chk1 [p Ser280] Antibody (SR1952)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22609

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-22609-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1952

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Chk1 Antibodies »

Product Summary for Chk1 [p Ser280] Antibody (SR1952)

Immunogen

A synthesized peptide derived from human phospho-Chk1 [Ser280] (Uniprot #: O14757)

Modification

p Ser 280

Specificity

Detects endogenous levels of total phospho-Chk1 [Ser280]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Chk1 [p Ser280] Antibody (SR1952)

Western Blot: Chk1 [p Ser 280] Antibody (SR1952) [NBP3-22609] -

Western Blot: Chk1 [p Ser 280] Antibody (SR1952) [NBP3-22609] - Western blot analysis using NBP3-22609 in 293T treated with Calyculin A cell lysate.

Applications for Chk1 [p Ser280] Antibody (SR1952)

Application
Recommended Usage

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Chk1

Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).

Long Name

Checkpoint Kinase 1

Alternate Names

CHEK1

Gene Symbol

CHEK1

Additional Chk1 Products

Product Documents for Chk1 [p Ser280] Antibody (SR1952)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Chk1 [p Ser280] Antibody (SR1952)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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