Key Product Details

Species Reactivity

Bacteria

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2/63

Concentration

1.0 mg/ml

View all Cholera Toxin Beta Antibodies »

Product Specifications

Immunogen

Vibrio cholerae

Localization

Secreted

Specificity

NB100-64675 detects the labile enterotoxin normally associated with V. cholera and reacts with an epitope on the B chain. No reactivity was observed with other species tested including various E. coli serotypes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Cholera Toxin Beta Antibody (2/63)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cholera Toxin Beta

The holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself. After binding to gangliosides GM1 in lipid rafts, through the subunit B pentamer, the holotoxin and the gangliosides are internalized. The holotoxin remains bound to GM1 until arrival in the ER. The A subunit has previously been cleaved in the intestinal lumen but the A1 and A2 chains have remained associated. In the ER, the A subunit disulfide bridge is reduced, the A1 chain is unfolded by the PDI and disassembled from the rest of the toxin. Then, the membrane-associated ER oxidase ERO1 oxidizes PDI, which releases the unfolded A1 chain. The next step is the retrotranslocation of A1 into the cytosol. This might be mediated by the protein-conducting pore SEC61. Upon arrival in the cytosol, A1 refolds and avoids proteasome degradation. In one way or another, A1 finally reaches its target and induces toxicity.

Alternate Names

Cholera enterotoxin B chain, Cholera enterotoxin beta chain, Cholera enterotoxin gamma chain, Cholera enterotoxin subunit B, Cholera toxin B protein, Choleragenoid, CTX B, CTXB, TOX B, toxB, VC1456

UniProt

P01556 (Bacteria)

Additional Cholera Toxin Beta Products

Product Documents for Cholera Toxin Beta Antibody (2/63)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cholera Toxin Beta Antibody (2/63)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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