Chordin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98689
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Chordin Antibody
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Chordin.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Chordin Antibody
Immunocytochemistry/ Immunofluorescence: Chordin Antibody [NBP2-98689]
Immunocytochemistry/Immunofluorescence: Chordin Antibody [NBP2-98689] - Immunofluorescence staining of Chordin in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Chordin polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(TM)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.Immunohistochemistry-Paraffin: Chordin Antibody [NBP2-98689]
Immunohistochemistry-Paraffin: Chordin Antibody [NBP2-98689] - Staining of human Chordin in human hepatoma with rabbit polyclonal antibody (1:1000).Immunocytochemistry/ Immunofluorescence: Chordin Antibody [NBP2-98689]
Immunocytochemistry/Immunofluorescence: Chordin Antibody [NBP2-98689] - Immunofluorescence staining of Chordin in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti- Chordin polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to nucleus.Applications for Chordin Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:300-1:10000
Immunohistochemistry-Paraffin
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Chordin
Alternate Names
CHRD, Sog
Gene Symbol
CHRD
Additional Chordin Products
Product Documents for Chordin Antibody
Product Specific Notices for Chordin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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