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Key Product Details

Species Reactivity

Human

Applications

Sandwich ELISA

Label

Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 77F12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with native human HCG (whole molecule).

Specificity

This specifically recognizes HCG; it reacts mainly with the alpha-subunit. It is cross-reacting with the whole molecule of HCG, hLH, hTSH and hFSH. Cross-reactivity with free beta-subunit of HCG was not detected.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications

Application
Recommended Usage

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Chorionic Gonadotropin alpha Chain (HCG alpha)

HCG is a glycoprotein hormone secreted in pregnancy by embryonic trophoblast cells during the first 7 weeks of gestation. Production subsequently shifts to the placenta, where it continues until term. HCG is a heterodimer consisting of an alpha chain and a beta chain. The alpha chain is common to several other hormones, including lutenizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH), while the beta chain is unique to HCG. Both alpha and beta chains contain a cysteine knot motif, also found in growth factors such as TGF-beta family members.

Alternate Names

alpha HCG, CGA, FSHA, GPHa1, LHA, TSHA

Gene Symbol

CGA

Additional Chorionic Gonadotropin alpha Chain (HCG alpha) Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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