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Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33064

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-33064

Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # AH21-10

Concentration

1 mg/ml

Product Summary for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10)

Immunogen

Recombinant protein within full length human Chorionic Gonadotropin alpha Chain (hCG alpha). (Uniprot: P01215)

Localization

Secreted.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10)

Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10)

Immunohistochemistry: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10) [NBP3-33064]

Immunohistochemistry: Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10) [NBP3-33064] - Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Mouse anti-Chorionic Gonadotropin alpha Chain (hCG alpha) antibody (NBP3-33064) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33064) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Chorionic Gonadotropin alpha Chain (HCG alpha)

HCG is a glycoprotein hormone secreted in pregnancy by embryonic trophoblast cells during the first 7 weeks of gestation. Production subsequently shifts to the placenta, where it continues until term. HCG is a heterodimer consisting of an alpha chain and a beta chain. The alpha chain is common to several other hormones, including lutenizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH), while the beta chain is unique to HCG. Both alpha and beta chains contain a cysteine knot motif, also found in growth factors such as TGF-beta family members.

Alternate Names

alpha HCG, CGA, FSHA, GPHa1, LHA, TSHA

Gene Symbol

CGA

Additional Chorionic Gonadotropin alpha Chain (HCG alpha) Products

Product Documents for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Chorionic Gonadotropin alpha Chain (hCG alpha) Antibody (AH21-10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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