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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat (Negative)

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

ELISA, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CGA/414

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant full-length human Chromogranin A protein (Uniprot: P10645)

Reactivity Notes

Does not react with Rat.

Mouse reactivity reported in scientific literature (PMID: 29352046).

Localization

Finely granular cytoplasmic

Marker

Neuroendocrine Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33198)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Chromogranin A Antibody (CGA/414)

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/414) [NBP2-29428]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/414) [NBP2-29428]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/414) [NBP2-29428] - Formalin-fixed, paraffin-embedded pancreas stained with Chromogranin A Ab (CGA414).
Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/414) [NBP2-29428]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/414) [NBP2-29428]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CGA/414) [NBP2-29428] - Formalin-fixed, paraffin-embedded human small cell Lung Carcinoma stained with Chromogranin A Monoclonal Antibody (CGA/414)

Applications for Chromogranin A Antibody (CGA/414)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Chromogranin A

Chromogranin A is found in secretory vesicles of neurons and endocrine cells. It is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Gene Symbol

CHGA

UniProt

Additional Chromogranin A Products

Product Documents for Chromogranin A Antibody (CGA/414)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Chromogranin A Antibody (CGA/414)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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