Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # LK2H10 + PHE5 + CGA/414

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Chromogranin A Antibodies »

Product Specifications

Immunogen

Human pheochromocytoma (LK2H10 & PHE5) & recombinant human chromogranin A protein (CGA414) (Uniprot: P10645)

Localization

Finely granular cytoplasmic

Marker

Neuroendocrine Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - IHC-Prediluted

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - IHC-Prediluted [NBP2-44772]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - IHC-Prediluted [NBP2-44772]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - IHC-Prediluted [NBP2-44772] - Paraffin-embedded human stomach tissue sections. pH 9 antigen retrieval. Image from verified customer review. Image using the Azide and BSA Free format of this antibody.
Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - IHC-Prediluted [NBP2-44772]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - IHC-Prediluted [NBP2-44772]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - IHC-Prediluted [NBP2-44772] - Formalin-fixed, paraffin-embedded human Adrenal Gland stained with Chromogranin A Monoclonal Antibody (LK2H10=PHE5+CGA414)
Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - IHC-Prediluted [NBP2-44772]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - IHC-Prediluted [NBP2-44772]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - IHC-Prediluted [NBP2-44772] - Formalin-fixed, paraffin-embedded human small cell lung carcinoma stained with Chromogranin A MAb (LK2H10+PHE5+CGA414)

Applications for Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.25 - 0.5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Chromogranin A

Chromogranin A (CgA) is an 86 kDa protein that is the major member of the granin family of acidic secretory glycoproteins located in neuroendocrine cells. CgA is believed to play a role in targeting peptide hormones and neurotransmitters to granules of the regulated pathways and inhibit hormone and neurotransmitter release. Also, the widespread distribution of CgA has made the measurement of circulating immunoreactive CgA a valuable tool in the diagnosis of neuroendocrine neoplasia, and CgA immunohistochemistry can help to identify the neuroendocrine nature of tumors. The N-terminal domain of CgA inhibits tumor necrosis factor a (TNFa) induced gap formation in human umbilical venous endothelial cells. CgA levels which reflect neuroendocrine differentiation of prostatic carcinoma may have a diagnostic, therapeutic and prognostic role in the management of prostate cancer patients.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Gene Symbol

CHGA

Additional Chromogranin A Products

Product Documents for Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - IHC-Prediluted

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Chromogranin A Antibody (LK2H10 + PHE5 + CGA/414) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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