Chromogranin B Antibody (JE31-72)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32186
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE31-72
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human Chromogranin B aa 601-650 / 677. (Uniprot: P05060)
Localization
Secreted.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Chromogranin B Antibody (JE31-72)
Western Blot: Chromogranin B Antibody (JE31-72) [NBP3-32186]
Western Blot: Chromogranin B Antibody (JE31-72) [NBP3-32186] - Western blot analysis of Chromogranin B on different lysates with Rabbit anti-Chromogranin B antibody (NBP3-32186) at 1/1,000 dilution.Lane 1: 293T cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: SW620 cell lysate
Lysates/proteins at 15 ug/Lane.
Predicted band size: 78 kDa
Observed band size: 120 kDa
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32186) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for Chromogranin B Antibody (JE31-72)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Chromogranin B
Alternate Names
CHGB, SCG1, Secretogranin I
Gene Symbol
CHGB
Additional Chromogranin B Products
Product Documents for Chromogranin B Antibody (JE31-72)
Product Specific Notices for Chromogranin B Antibody (JE31-72)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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