Chromogranin B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80782

Novus Biologicals, part of Bio-Techne
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NBP1-80782
NBP1-80782-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Chromogranin B Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KQYSSHHTAEKRKRLGELFNPYYDPLQWKSSHFERRDNMNDNFLEGEEENELTLNEKNFFPEYNYDWWEKKPFSEDVNWGYEKRNLARVPKLDLKRQYDRVAQLDQLLHYRKKSAEFPDFYDS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Chromogranin B Antibody

Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782]

Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782]

Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Analysis in human adrenal gland and liver tissues using NBP1-80782 antibody. Corresponding CHGB RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782]

Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782]

Immunohistochemistry-Paraffin: Chromogranin B Antibody [NBP1-80782] - Staining of human adrenal gland, colon, liver and pancreas using Anti-CHGB antibody NBP1-80782 (A) shows similar protein distribution across tissues to independent antibody NBP1-80781 (B).
Western Blot: Chromogranin B Antibody [NBP1-80782]

Western Blot: Chromogranin B Antibody [NBP1-80782]

Western Blot: Chromogranin B Antibody [NBP1-80782] - Analysis in human cell line SH-SY5Y.
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Applications for Chromogranin B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Chromogranin B

The Chromogranin B gene encodes a tyrosine-sulfated secretory protein abundant in peptidergic endocrine cells and neurons. Thisprotein may serve as a precursor for regulatory peptides. (provided by RefSeq)

Alternate Names

CHGB, SCG1, Secretogranin I

Entrez Gene IDs

1114 (Human)

Gene Symbol

CHGB

UniProt

P05060

Additional Chromogranin B Products

Product Documents for Chromogranin B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Chromogranin B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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