ChT1 antigen Antibody (CT1) [PE]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28358

Novus Biologicals, part of Bio-Techne
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NBP1-28358
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Key Product Details

Species Reactivity

Avian, Chicken

Applications

Flow Cytometry, Immunoprecipitation, In vitro assay

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CT1

Concentration

0.1 mg/ml

View all ChT1 antigen Antibodies »

Product Specifications

Immunogen

Chicken thymocytes

Specificity

Chicken/Quail ChT1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ChT1 antigen Antibody (CT1) [PE]

Flow Cytometry: ChT1 antigen Antibody (CT1) [PE] [NBP1-28358] - Chicken thymocytes were double stained with mouse anti-chicken CD3-PE and mouse antichicken ChT1-FITC. Analysis was performed on a Flow cytometer using a light scatter gate to identify small lymphocytes.

Applications for ChT1 antigen Antibody (CT1) [PE]

Application
Recommended Usage

Flow Cytometry

<= 0.2 ug/10^6 cells

Immunoprecipitation

1:10-1:500

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS and a stabilizing agent

Preservative

Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ChT1 antigen

ChT1, a member of the Ig superfamily with one V-like and one C2-like domain, is a T cell antigen that is expressed on the surface of embryonic thymocytes (day 10). In young chickens, about 90% of the thymocytes as well as a subpopulation of peripheral lymphocytes (which represent recent thymic emigrants) are ChT1 positive.1-4 Expression in the periphery declines with age and, in correlation with partial thymectomy, indicates that ChT1 can be used as an accurate marker for studying thymic function. (3) CT1 can block T cell differentiation in vitro, in thymic organ cultures and in thymocyte precursors cultured on stromal cell monolayers. (4) CT1 also recognizes quail cortical thymocytes. (1)

Alternate Names

ChT1 thymocyte antigen, V set and immunoglobulin domain containing, VSIG1

Additional ChT1 antigen Products

Product Documents for ChT1 antigen Antibody (CT1) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ChT1 antigen Antibody (CT1) [PE]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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