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CHX10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84476

Novus Biologicals, part of Bio-Techne
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NBP1-84476
NBP1-84476-25ul

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Predicted:

Mouse (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LGLNKEPPSSHPRAALDGLAPGHLLAARSVLSPAGVGGMGLLGPGGLPGFYTQPTFLEVLSDPQSVHLQPLGRASGPLDTSQTASSDSEDVSSSDRKMSKSALNQTKKRKKRRHRTIFTSYQLEELEKAFNEAH

Marker

pan-Bipolar Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHX10 Antibody

Western Blot: CHX10 Antibody [NBP1-84476]

Western Blot: CHX10 Antibody [NBP1-84476]

Western Blot: CHX10 Antibody [NBP1-84476] - Analysis in control (vector only transfected HEK293T lysate) and vSX2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: CHX10 Antibody [NBP1-84476]

Immunohistochemistry-Paraffin: CHX10 Antibody [NBP1-84476]

Immunohistochemistry-Paraffin: CHX10 Antibody [NBP1-84476] - Staining of human skeletal muscle shows no nuclear positivity in myocytes as expected.
Immunohistochemistry-Paraffin: CHX10 Antibody [NBP1-84476]

Immunohistochemistry-Paraffin: CHX10 Antibody [NBP1-84476]

Immunohistochemistry-Paraffin: CHX10 Antibody [NBP1-84476] - Staining of human retina shows strong nuclear positivity in inner nuclear layer.

Applications for CHX10 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHX10

The CHX10 gene encodes a homeobox protein originally described as a retina-specific transcription factor. Mutations in thisgene are associated with microphthalmia, cataracts and iris abnormalities. (provided by RefSeq)

Alternate Names

ceh-10 homeo domain containing homolog, ceh-10 homeo domain containing homolog (C. elegans), ceh-10 homeodomain containing homolog (C. elegans), Ceh-10 homeodomain-containing homolog, CHX10MCOPCB3, Homeobox protein CHX10, HOX10C elegans ceh-10 homeo domain-containing homolog, MCOP2, RET1, visual system homeobox 2

Entrez Gene IDs

338917 (Human)

Gene Symbol

VSX2

UniProt

Additional CHX10 Products

Product Documents for CHX10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CHX10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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