Chymotrypsin-like protease Antibody (10Z4K3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15845
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: Chymotrypsin-like protease Antibody (10Z4K3) [NBP3-15845]](https://resources.bio-techne.com/images/products/Chymotrypsin-like-protease-Antibody-10Z4K3-Immunocytochemistry-Immunofluorescence-NBP3-15845-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Chymotrypsin-like protease Antibody (10Z4K3) [NBP3-15845]")
![Immunohistochemistry-Paraffin: Chymotrypsin-like protease Antibody (10Z4K3) [NBP3-15845]](https://resources.bio-techne.com/images/products/Chymotrypsin-like-protease-Antibody-10Z4K3-Immunohistochemistry-Paraffin-NBP3-15845-img0001.jpg "Immunohistochemistry-Paraffin: Chymotrypsin-like protease Antibody (10Z4K3) [NBP3-15845]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10Z4K3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Chymotrypsin-like protease Antibody (10Z4K3)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 165-264 of human Chymotrypsin-like protease (P40313). GVGNVTPAHLQQVALPLVTVNQCRQYWGSSITDSMICAGGAGASSCQGDSGGPLVCQKGNTWVLIGIVSWGTKNCNVRAPAVYTRVSKFSTWINQVIAYN
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Chymotrypsin-like protease Antibody (10Z4K3)
![Western Blot: Chymotrypsin-like protease Antibody (10Z4K3) [NBP3-15845]](https://resources.bio-techne.com/images/products/Chymotrypsin-like-protease-Antibody-10Z4K3-Western-Blot-NBP3-15845-img0005.jpg "Western Blot: Chymotrypsin-like protease Antibody (10Z4K3) [NBP3-15845]")
Western Blot: Chymotrypsin-like protease Antibody (10Z4K3) [NBP3-15845]
Western Blot: Chymotrypsin-like protease Antibody (10Z4K3) [NBP3-15845] - Western blot analysis of extracts of various cell lines, using Chymotrypsin-like protease Rabbit mAb (NBP3-15845) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunocytochemistry/ Immunofluorescence: Chymotrypsin-like protease Antibody (10Z4K3) [NBP3-15845]](https://resources.bio-techne.com/images/products/Chymotrypsin-like-protease-Antibody-10Z4K3-Immunocytochemistry-Immunofluorescence-NBP3-15845-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Chymotrypsin-like protease Antibody (10Z4K3) [NBP3-15845]")
Immunocytochemistry/ Immunofluorescence: Chymotrypsin-like protease Antibody (10Z4K3) [NBP3-15845]
Immunocytochemistry/Immunofluorescence: Chymotrypsin-like protease Antibody (10Z4K3) [NBP3-15845] - Immunofluorescence analysis of mouse pancreas cells using Chymotrypsin-like protease Rabbit mAb (NBP3-15845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: Chymotrypsin-like protease Antibody (10Z4K3) [NBP3-15845]](https://resources.bio-techne.com/images/products/Chymotrypsin-like-protease-Antibody-10Z4K3-Immunohistochemistry-Paraffin-NBP3-15845-img0002.jpg "Immunohistochemistry-Paraffin: Chymotrypsin-like protease Antibody (10Z4K3) [NBP3-15845]")
Immunohistochemistry-Paraffin: Chymotrypsin-like protease Antibody (10Z4K3) [NBP3-15845]
Immunohistochemistry-Paraffin: Chymotrypsin-like protease Antibody (10Z4K3) [NBP3-15845] - Immunohistochemistry of paraffin-embedded mouse pancreas using Chymotrypsin-like protease Rabbit mAb (NBP3-15845) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for Chymotrypsin-like protease Antibody (10Z4K3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:5000 - 1:8000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Chymotrypsin-like protease
Alternate Names
chymotrypsin-like, chymotrypsin-like protease CTRL-1, CTRL1, EC 3.4.21, EC 3.4.21.-, MGC70821
Gene Symbol
CTRL
Additional Chymotrypsin-like protease Products
Product Documents for Chymotrypsin-like protease Antibody (10Z4K3)
Product Specific Notices for Chymotrypsin-like protease Antibody (10Z4K3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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