Skip to main content

CIAO1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84474

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP1-84474
NBP1-84474-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CSDDRTVRIWRQYLPGNEQGVACSGSDPSWKCICTLSGFHSRTIYDIAWCQLTGALATACGDDAIRVFQEDPNSDPQQPTFSLTAHLHQAHSQDVNCVAWNPKEPGLLASCSDDGEVAFWKYQR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CIAO1 Antibody

Western Blot: CIAO1 Antibody [NBP1-84474]

Western Blot: CIAO1 Antibody [NBP1-84474]

Western Blot: CIAO1 Antibody [NBP1-84474] - Analysis in control (vector only transfected HEK293T lysate) and CIAO1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: CIAO1 Antibody [NBP1-84474]

Immunocytochemistry/ Immunofluorescence: CIAO1 Antibody [NBP1-84474]

Immunocytochemistry/Immunofluorescence: CIAO1 Antibody [NBP1-84474] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: CIAO1 Antibody [NBP1-84474]

Immunohistochemistry-Paraffin: CIAO1 Antibody [NBP1-84474]

Immunohistochemistry-Paraffin: CIAO1 Antibody [NBP1-84474] - Staining of human oral mucosa shows nuclear positivity in squamous epithelial cells.

Applications for CIAO1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CIAO1

Essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins. Seems to specifically modulate the transactivation activity of WT1

Alternate Names

CIA1, cytosolic iron-sulfur protein assembly 1, cytosolic iron-sulfur protein assembly 1 homolog (S. cerevisiae), probable cytosolic iron-sulfur protein assembly protein CIAO1, WD repeat domain 39, WD repeat-containing protein 39, WD40 protein Ciao1, WDR39cytosolic iron-sulfur protein assembly 1 homolog

Gene Symbol

CIAO1

Additional CIAO1 Products

Product Documents for CIAO1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CIAO1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...