CIAO1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84474
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (94%), Rat (92%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CSDDRTVRIWRQYLPGNEQGVACSGSDPSWKCICTLSGFHSRTIYDIAWCQLTGALATACGDDAIRVFQEDPNSDPQQPTFSLTAHLHQAHSQDVNCVAWNPKEPGLLASCSDDGEVAFWKYQR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CIAO1 Antibody
Western Blot: CIAO1 Antibody [NBP1-84474]
Western Blot: CIAO1 Antibody [NBP1-84474] - Analysis in control (vector only transfected HEK293T lysate) and CIAO1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: CIAO1 Antibody [NBP1-84474]
Immunocytochemistry/Immunofluorescence: CIAO1 Antibody [NBP1-84474] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.Immunohistochemistry-Paraffin: CIAO1 Antibody [NBP1-84474]
Immunohistochemistry-Paraffin: CIAO1 Antibody [NBP1-84474] - Staining of human oral mucosa shows nuclear positivity in squamous epithelial cells.Applications for CIAO1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500-1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CIAO1
Alternate Names
CIA1, cytosolic iron-sulfur protein assembly 1, cytosolic iron-sulfur protein assembly 1 homolog (S. cerevisiae), probable cytosolic iron-sulfur protein assembly protein CIAO1, WD repeat domain 39, WD repeat-containing protein 39, WD40 protein Ciao1, WDR39cytosolic iron-sulfur protein assembly 1 homolog
Gene Symbol
CIAO1
Additional CIAO1 Products
Product Documents for CIAO1 Antibody
Product Specific Notices for CIAO1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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