Cingulin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89600

Novus Biologicals, part of Bio-Techne
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NBP1-89600
NBP1-89600-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cingulin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DSKQALQQLQAQLEDYKEKARREVADAQRQAKDWASEAEKTSGGLSRLQDEIQRLRQALQASQAERDTARLDKELLAQRLQGLEQEAENKKRSQDDRARQLKGLEE

Reactivity Notes

Immunogen shows 92% sequence identity with mouse, however WB testing using NIH/3T3 cells shows no positive results.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cingulin Antibody

Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89600]

Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89600]

Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89600] - Staining in human small intestine and lymph node tissues using anti-CGN antibody. Corresponding CGN RNA-seq data are presented for the same tissues.
Western Blot: Cingulin Antibody [NBP1-89600]

Western Blot: Cingulin Antibody [NBP1-89600]

Western Blot: Cingulin Antibody [NBP1-89600] - Analysis using Anti-CGN antibody NBP1-89600 (A) shows similar pattern to independent antibody NBP1-89602 (B).
Western Blot: Cingulin Antibody [NBP1-89600]

Western Blot: Cingulin Antibody [NBP1-89600]

Western Blot: Cingulin Antibody [NBP1-89600] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
View 3 more images

Applications for Cingulin Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 31719823).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cingulin

Alternate Names

cingulin, DKFZp779N1112, KIAA1319

Gene Symbol

CGN

Additional Cingulin Products

Product Documents for Cingulin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cingulin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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