CISD1 Antibody (1A8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22567

Novus Biologicals, part of Bio-Techne
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NBP2-22567
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1A8

Format

BSA Free

Concentration

1 mg/ml

View all CISD1 Antibodies »

Product Summary for CISD1 Antibody (1A8) - BSA Free

Immunogen

Recombinant human CISD1 (32-108aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CISD1 Antibody (1A8) - BSA Free

Western Blot: CISD1 Antibody (1A8)BSA Free [NBP2-22567]

Western Blot: CISD1 Antibody (1A8)BSA Free [NBP2-22567]

Western Blot: CISD1 Antibody (1A8) [NBP2-22567] - The cell lysates (40ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-humanCISD1 antibody (1:1000). Proteins were visualized using agoat anti-mouse secondary antibody conjugated to HRP andan ECL detection system.Lane 1.: K562 cell lysateLane 2.: MCF7 cell lysat
Flow Cytometry: CISD1 Antibody (1A8) - BSA Free [NBP2-22567]

Flow Cytometry: CISD1 Antibody (1A8) - BSA Free [NBP2-22567]

Flow Cytometry: CISD1 Antibody (1A8) [NBP2-22567] - Analysis of CISD1 in THP-1 cell line, stainingat 2-5ug for 1x106cells (red line). The secondary antibodyused goat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).
Western Blot: CISD1 Antibody (1A8)BSA Free [NBP2-22567]

Western Blot: CISD1 Antibody (1A8)BSA Free [NBP2-22567]

Western Blot: CISD1 Antibody (1A8) [NBP2-22567] - The extract of Mouse liver (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody.

Applications for CISD1 Antibody (1A8) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CISD1

CISD1 (CDGSH iron-sulfur domain-containing protein 1) is 108 amino acid protein. CISD1 plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation. May be involved in Fe-S cluster shuttling and/or in redox reactions. This protein expression is reduced in cells derived from cystic fibrosis patients.

Alternate Names

C10orf70, CDGSH iron sulfur domain 1, CDGSH iron sulfur domain-containing protein 1, CDGSH iron-sulfur domain-containing protein 1, chromosome 10 open reading frame 70, MDS029, MGC14684, mitoNEET, ZCD1, zinc finger CDGSH-type domain 1, zinc finger, CDGSH-type domain 1

Gene Symbol

CISD1

UniProt

NP_060934 (Human)

Additional CISD1 Products

Product Documents for CISD1 Antibody (1A8) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CISD1 Antibody (1A8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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