CISD1 Antibody (OTI2B3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72024

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2B3

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CISD1 Antibodies »

Product Summary for CISD1 Antibody (OTI2B3) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human CISD1 (NP_060934) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CISD1 Antibody (OTI2B3) - Azide and BSA Free

Western Blot: CISD1 Antibody (OTI2B3)Azide and BSA Free [NBP2-72024]

Western Blot: CISD1 Antibody (OTI2B3)Azide and BSA Free [NBP2-72024]

Western Blot: CISD1 Antibody (OTI2B3) - Azide and BSA Free [NBP2-72024] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CISD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CISD1.
Immunocytochemistry/ Immunofluorescence: CISD1 Antibody (OTI2B3) - Azide and BSA Free [NBP2-72024]

Immunocytochemistry/ Immunofluorescence: CISD1 Antibody (OTI2B3) - Azide and BSA Free [NBP2-72024]

Immunocytochemistry/Immunofluorescence: CISD1 Antibody (OTI2B3) - Azide and BSA Free [NBP2-72024] - Staining of HeLa cells using anti-CISD1 mouse monoclonal antibody .
Flow Cytometry: CISD1 Antibody (OTI2B3) - Azide and BSA Free [NBP2-72024]

Flow Cytometry: CISD1 Antibody (OTI2B3) - Azide and BSA Free [NBP2-72024]

Flow Cytometry: CISD1 Antibody (OTI2B3) - Azide and BSA Free [NBP2-72024] - Analysis of Jurkat cells, using anti-CISD1 antibody , (Red), compared to a nonspecific negative control antibody , (Blue).
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Applications for CISD1 Antibody (OTI2B3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CISD1

CISD1 is a member of the CDGSH domain-containing family and may play a role in the regulation of mitochondrial oxidative capacity (Wiley et al., 2007, 2007 [PubMed 17376863] [PubMed 17584744]).[supplied by OMIM]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.

Alternate Names

C10orf70, CDGSH iron sulfur domain 1, CDGSH iron sulfur domain-containing protein 1, CDGSH iron-sulfur domain-containing protein 1, chromosome 10 open reading frame 70, MDS029, MGC14684, mitoNEET, ZCD1, zinc finger CDGSH-type domain 1, zinc finger, CDGSH-type domain 1

Gene Symbol

CISD1

Additional CISD1 Products

Product Documents for CISD1 Antibody (OTI2B3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CISD1 Antibody (OTI2B3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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