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CISD2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84809

Novus Biologicals, part of Bio-Techne
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NBP1-84809

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PKKKQQKDSLINLKIQKENPKVVNEINIEDLCLTKAAYCRCWRSKTFPACDGSHNKHNELTGDNVGPLILKKKEV

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CISD2 Antibody

Western Blot: CISD2 Antibody [NBP1-84809]

Western Blot: CISD2 Antibody [NBP1-84809]

Western Blot: CISD2 Antibody [NBP1-84809] - Analysis in control (vector only transfected HEK293T lysate) and CISD2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: CISD2 Antibody [NBP1-84809]

Immunocytochemistry/ Immunofluorescence: CISD2 Antibody [NBP1-84809]

Immunocytochemistry/Immunofluorescence: CISD2 Antibody [NBP1-84809] - Staining of human cell line U-251 MG shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CISD2 Antibody [NBP1-84809]

Immunohistochemistry-Paraffin: CISD2 Antibody [NBP1-84809]

Immunohistochemistry-Paraffin: CISD2 Antibody [NBP1-84809] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Applications for CISD2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CISD2

Alternate Names

CDGSH iron sulfur domain 2, CDGSH iron-sulfur domain-containing protein 2, CDGSH2, Endoplasmic reticulum intermembrane small protein, ERISWFS2, Miner1Wolfram syndrome 2, mitoNEET related 1, MitoNEET-related 1 protein, NAF-1, Nutrient-deprivation autophagy factor-1, ZCD2CDGSH iron sulfur domain-containing protein 2, zinc finger, CDGSH-type domain 2

Gene Symbol

CISD2

Additional CISD2 Products

Product Documents for CISD2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CISD2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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