CIZ1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33890

Novus Biologicals, part of Bio-Techne
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NBP2-33890
NBP2-33890-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CIZ1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: EQTPVVVHVCGLEMPPDAVEAGGGMEKTLPEPVGTQVSMEEIQNESACGLDVGECENRAREMPGVWGAGGSLKVTIL

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27163549).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CIZ1 Antibody

Immunocytochemistry/ Immunofluorescence: CIZ1 Antibody [NBP2-33890]

Immunocytochemistry/ Immunofluorescence: CIZ1 Antibody [NBP2-33890]

Immunocytochemistry/Immunofluorescence: CIZ1 Antibody [NBP2-33890] - Staining of human cell line MCF7 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: CIZ1 Antibody [NBP2-33890]

Immunohistochemistry-Paraffin: CIZ1 Antibody [NBP2-33890]

Immunohistochemistry-Paraffin: CIZ1 Antibody [NBP2-33890] - Staining of human vulva/anal skin shows strong nuclear positivity in epidermal cells.
CIZ1 Antibody

Immunohistochemistry-Paraffin: CIZ1 Antibody [NBP2-33890] -

Immunohistochemistry-Paraffin: CIZ1 Antibody [NBP2-33890] - Staining of human testis shows moderate nuclear positivity in spermatids.
View 2 more images

Applications for CIZ1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

Reactivity reported in (PMID: 27163549)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CIZ1

CIZ1 (Cip1 interacting zinc-finger protein) was identified as a protein that associates with the cell-cycle inhibiting protein, p21 (Cip1/Waf1). CIZ1 is a member of the matrin 3 protein family and has been shown to bind DNA consensus sequences and co-localize with PCNA during replication. In breast cancer cells, CIZ1 was shown to associate with Cdk2 and dynein light chain 1 (DLC1), a cytoskeletal signaling protein involved in cell cycle regulation and tumorigenesis. Studies of the role of CIZ1 in breast tumorigenesis revealed that CIZ1 is an estrogen-responsive gene that functions as a coactivator of the estrogen receptor. Targeted depletion of CIZ1 inhibits entry into S-phase. This finding along with evidence that CIZ1 interacts with multiple cell cycle regulatory proteins and localizes to DNA replication foci, indicates that CIZ1 plays a critical role in the regulation of DNA replication.

Alternate Names

CDKN1A interacting zinc finger protein 1, CDKN1A-interacting zinc finger protein 1, cip1-interacting zinc finger protein, LSFR1ZNF356Nuclear protein NP94, NP94, Zinc finger protein 356

Gene Symbol

CIZ1

UniProt

Q9ULV3 (Human)

Additional CIZ1 Products

Product Documents for CIZ1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CIZ1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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